NEWSLETTER

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P.O. Box 400 - New Hyde Park, N.Y. 11040

E-mail: newsletter@alken-murray.com

Websites: http://www.alken-murray.com 
 http://www.clear-flo.com

 http://www.even-flo.com

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 Vol. 6: Issue No. 2

 Fall 2002

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General News
Alken 896 - Revised MSDS and product bulletin
Updated information on this oxygenated nitrate remediation accelerant

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Alken® Microbial Technical Updates
Alken Clear-Flo 1005 and Alken Enz-Odor 5 Improved
We have recently added Starkeya novella, sulfide, disulfide and mercaptan oxidizer to our arsenal of microbes in Alken Clear-Flo 1005 and Alken Enz-Odor 5. We are studying the possibility of adding this strain to other products in the future. This facultative chemolithoautotrophic microbe was formerly known as Thiobacillus novellus, but the DNA indicates that it is not really genetically close to the Thiobacillus family, although it is a gram-negative, colorless sulfur microbe. We also offer this strain alone for the same price as our other 5 billion count Alken Clear-Flo 7000 line products, for those who like to customize their own formulas, under the name Alken Enz-Odor 14.
Alken NuBind 2 - Improved
We have increased stability, scent, surfactant properties, and have increased the total count of this product to our standard for liquids of 54 million CFU/ml.
Alken Clear-Flo 7139 - R & D product available for testing
A new soil-remediation blend includes our successful gram-positive spore-forming, microbe consortium from Clear-Flo 5100, and our high molecular weight humified peat extract from Alken Nu-Bind with the catalysts and extra nutrients from Alken Clear-Flo 5100. Available for testing.
Alken Clear-Flo 5300 - New Product
New product created for a dentist to clean the vacuum lines from his aspiration pumps, better than alternative methods with greater safety in handling than caustic cleaners. Includes non-pathogenic waste-degrading microbes from our original Alken Enz-Odor formulation, combined with metasilicate and specialized surfactants from Alken Surface Cleaner 874 and Alken Antifoam 880, to eliminate foaming.
New Use - Alken Nu-Bind (Original)
Reduce clumping of filamentous algae with Alken Nu-Bind, to augment your standard treatment with Alken Clear-Flo 1005 for sludge remediation and Alken Clear-Flo 1006 for water clarify and probiotic effects on aquatic inhabitants.
Second New Use for Alken Nu-Bind
Alken Nu-Bind
has now been proven to knock foam and break up the clumping effect of Nocardia and other foam-causing filamentous organisms, if sprayed heavily on top of it. When used in combination with Alken Clear-Flo 7015, which should be applied at the front of the plant to digest excess surfactants, food fats and grease, control can be gained in shorter time than when Alken Clear-Flo 7015 is used alone. Our first client to test this treatment is willing to discuss his application techniques and product performance with potential clients. We hope to have details soon for a written case study. In the meantime, call him at City of Gatesville waste treatment facility.
Independent Tests Praise Alken Clear-Flo 7001 and 7110-50x, Alken 896 & Alken Nu-Bind
Independent tests performed in Kuwait, evaluated and approved Alken Clear-Flo 7001, Alken 896, Alken Nu-Bind (original) and Alken Clear-Flo 7110-50x. They also tested Alken Nu-Bind and Alken Clear-Flo 7001 together and discovered that they interfere with each other, so recommend they be applied separately in systems. We are now also recommending that Nu-Bind not be mixed with any other product before application, but rather, apply separately.
New Case Study - Sausage Factory in Europe
Thank you Alina and Sotiris of BioBest, our Greek distributor, for a new case study demonstrating the use of Alken Clear-Flo blends in a large sausage factory, bringing the plant into compliance in less than two weeks and giving even better results by 3 weeks, allowing reduced dosages to maintain compliant performance. Our Greek distributor has promised us five more waste treatment case studies soon.
Enzymes to Avoid in Odor Control Formulations
The urease enzyme turns urine into ammonia, so Alken-Murray tries to avoid this enzyme in our microbes, unless other talents overwhelm this drawback. We also try to avoid microbes that rapidly utilize citrate and L-lysine because the daughter-products include ammonia. We tested a commonly used competitor's 5-strain blend by adding those microbes to our other Alken Enz-Odor and Alken Enz-Odor 4 ingredients, to provide a fair comparison with our formulations, immediately after the Fenton Farm, Inc. cattery (part of our research facility) was completely cleaned. Within a few hours of adding the substituted dry formula to the litterpans and spraying the substituted odor control liquid onto areas marked by our stud cats, the resulting release of ammonia in the cattery was so overpowering that we were forced to change all litterpans immediately and scrub the floor and walls with a diluted chlorine bleach solution to eliminate the odor. See our enzyme article for further information on enzymes.
MTBE
We have had good results degrading MTBE with Alken Clear-Flo 7038, when it is part of gasoline or with Alken Clear-Flo 7036, when it is part of a mixed fuel spill, but we do not treat this substance ALONE, once it has escaped to the water table. The daughter-products are hazardous enough that our board of directors decided that we should not take on the huge liability attached to entering into treating contaminated water tables.
Revised QC-3 Salmonella test Procedure
Hardy Diagnostics
offers Chromagar Salmonella , which displays mauve colonies when positive for Salmonella and blue, colorless or white for other species. No enrichment is needed when testing microbe products, but if you are testing wastewater, you may need to enrich the culture first to get a higher count. See more information at http://www.chromagar.com.
Revised QC-8 - Procedure to Verify Freedom from Staphylococcus
This procedure is designed to verify that all Alken Clear-Flo® products are free of Staphylococcus aureus. The primary presumptive European test for Staphylococcus is Chapman's Agar, the equivalent of our Mannitol Salt Agar (QC-21), which does not distinguish between Staphylococcus and many of Alken-Murray's gram-positive, salt loving, Mannitol utilizing Bacillus strains. For initial identification, use CHROMagar Staphylococcus. For verification, you can also use Chromagar Aureole, on which Staphylococcus aureus shows up as black colonies with a white halo.
Revised QC-75 - Oxoid BCET-RPLA Enterotoxin Test Procedure
We found it a bit difficult to follow the procedure in their product insert, due to small type size, so we have written our own version of their instructions, including an especially prominent warning against making bubbles during the dilution procedure, as this can interfere with results.
Revised QC-101 Aeromonas Identification
Hardy Diagnostics
offers a Blood agar with Ampicillin, sold as product A12, for the rapid identification of Aeromonas. You can use the same counting procedure detailed in their literature for Chromagar ECC to estimate the count of Aeromonas in a pond. This is a faster and easier procedure than using a series of tests that were formerly required to distinquish Aeromonas from other gram-negative strains that are similar. Aeromonas is immune to Ampicillin, so it should be the only strain found in a pond that will grow on that media.
Newly Posted - Alken-Murray's Treatise on Swine Manure Management
Known among Alken-Murray distributors as "the 2 lagoon paper", this information was not posted until now. We hope you enjoy this information, which can be applied to other types of waste treatment as well.
Research and Development
We are getting closer to bringing our fabulous new AMH series of strains, isolated from humified peat soil, to the marketplace. Three strains have passed initial commercial scale-up and should be added to products soon. Three more strains have passed lab scale-up and have been approved for commercial growth in the next few months. Three of the AMH strains will be the focus of a scientific paper, written in conjunction with Dr. Frederick Cohan of Wesleyan University, detailing their unique features and officially naming them as a new species of Bacillus. We also have two more low pH strains isolated from pine forest soil, that have passed scale-up tests and will be added to products in 2003. We plan to introduce at least 10 new products and improve many others, so stay tuned to this occasionally published newsletter for updates.

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Fuel Treatment Technical Updates
Fireside Additives Deleted
We have eliminated Alken Fireside 944 and 945, Alken 935 and Alken Fireside 981 because of difficulty with quality control for these products and minimal requests for these products. The Alken Even-Flo wholly-organic formulas work better, less expensively and have less DOT transport restrictions. All Alken Even-Flo fuel treatments will still be available from us.

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