Effective Date: 03/21/2007

 Product Bulletin

 Material Safety Data Sheet
 Manufacturer's Name: Alken-Murray Corporation
 Manufacturer's Address: P.O. Box 400, New Hyde Park, New York 11040
  T: 540-636-1236 Fax: 540-636-1770
 Manufacturer's Cage Code: 2A384
For chemical emergency - spill, leak, fire or accident - call CHEMTREC - day or night - (800) 424-9300 or outside the USA (703) 527-3887

NFPA Hazard Ratings:

0=Minimal, 1=Slight, 2=Moderate, 3=Serious, 4=Severe

 Health=1  Fire=0  Reactivity=0

Canadian WHMIS Classification D 2 B

All ingredients on Canadian DSL and USA TSCA lists

1. Product Identification

 Synonyms:  Aquaculture Probiotic Transport Probiotic Aid
 CAS Number:  Not applicable to mixtures
 Chemical Formula:  Proprietary Mixture
 Formula ID Codes:  1400

2. Composition/Information on Ingredients


Ingredients not precisely identified are proprietary and/or non-hazardous per 29 CFR 1910-1200 and/or represent less than 1%. All chemical ingredients appear on the EPA TSCA inventory.

 Chemical Name  CAS Number  OSHA PEL  ACGIH TLV   % Weight
 Water  7732-18-5   NDA   NDA  > 95
 Sodium bicarbonate  144-55-8  NDA  NDA  < 2
 Viable bacterial cultures  NA  NDA   NDA   1
Glycerine  56-81-5   NDA  NDA  < 1
 Sodium hydroxide    1310-73-2  2 mg/M3  2 mg/M3   < 1 
 Ammonium sulfate  7783-20-2  NDA  NDA    Trace
 Magnesium sulfate  7487-88-9  NDA  NDA  Trace
 Calcium chloride  10043-52-4  NDA  NDA  Trace
 Disodium phosphate (DSP)  7558-79-4   15 mg/m3   10 mg/m3  Trace
 Ferric EDTA, sodium salt  15708-41-5  NDA  NDA  Trace
Sodium molybdate dihydrate  7631-95-0  5 mg/m3  5 mg/m3  Trace
Manganese sulfate  10034-96-5    NDA   NDA  Trace
 Cobalt chloride  7791-13-1   NDA   NDA  Trace
 Zinc sulfate  7446-20-0   NDA   NDA  Trace
 EPPS buffer  16052-06-5   NDA   NDA  Trace

3. Hazards Identification

 Emergency Overview: The microorganisms in this product are all rated BioSafety Class 1, as defined by the Centers for Disease Control, species known to be unlikely to cause disease in healthy humans,animals or plants. Two strains of autotrophic nitrifiers and three strains of endospore-forming Bacillus are susceptible to the following antibiotics, listed following strain identification by Alken-Murray "house ID", which follows the genus and species name for each strain that is part of this formula.

  • Nitrosomonas europaea, strain 978 , is sensitive to Cephalothin, Ceftriaxone, Streptomycin, Chloramphenicol, Erythromycin, Penicillin G., Kanamycin and Trimethoprim/Sulfoxazole.
  • Nitrobacter winogradskyi, strain 391 , is sensitive to Cephalothin, Ceftriaxone, Streptomycin, Chloramphenicol, Erythromycin, Penicillin G., Kanamycin and Trimethoprim/Sulfoxazole.
  • Bacillus licheniformis, strain 406, is sensitive to Amoxicillin/Clavulanic acid, Cephalothin, Erythromycin, Gentamicin, Oxacillin, Streptomycin, Tetracycline, Trimethoprim/Sulfa, and Vancomycin.
  • Bacillus mojavensis, strain AMH 100, is sensitive to Ampicillin, Cephalothin, Gentamicin. Tetracycline. Clindamycin, Penicillin, & Vancomycin. Resistant to Ceftriaxone, Chloramphenicol, Erythromycin and Lincomycin.
  • Bacillus subtilis, strain 405, is sensitive to Amikacin, Ampicillin, Ceftriaxone, Chloramphenicol, Gentamicin, Mezocillin, Streptomycin, Tobramycin and Carbenicillin.


The pair of authotrophic nitrifier strains, the first two strains listed above, were grown in a sterile aqueous medium containing nutrients identified in section 2 of this MSDS, but following fermentation, ultra-filtration (designed to increase concentration of bacterial cultures, while discarding excess water and micro-nutrients to minimize total volume and weight). The Bacillus cultures, the trio of strains listed above, following the pair of nitrifiers, were also grown in a sterile aqueous medium containing a variety of nutrients, but as heterotrophic bacteria, nutrients used include corn syrup solids, digests of soybean and/or skim milk, glucose or other sugars, vegetable oils, including olive oil, yeast extract, butterfat, starch, mineral salts, etc. The residual concentrations of these nutrients are believed to be below levels capable of triggering irritation, which also assures that product cultures will switch over to using the tank, pond or lake pollution as their sole nutrition, within six to twelve hours after hydration and application.

Access the antibiotic sensitivity chart at , which provides results of Kirby-Bauer antibiotic sensitivity screening of Alken-Murray's strains, on a strain by strain basis. See more about Alken-Murray's policies in that document.

Potential Health Effects:

  • Signs and Symptoms of Acute Overexposure: Eye, skin irritation
  • Signs and Symptoms of Chronic overexposure: Dermatitis and skin sensitization
  • Medical Conditions Generally Aggravated by Exposure: Allergies, dermatitis.
  • Ingestion: No effect if ingested in small amounts. Relative to other materials, a single dose of this product is rarely toxic by ingestion. Irritation of the mouth, pharynx, esophagus and stomach can develop following ingestion.

4. First Aid Measures

  • Inhalation: Remove victim to fresh air
  • Ingestion: Give victim water or milk to drink to dilute the product. Get medical advice..
  • Skin Contact: Remove contaminated clothing and footwear. Wash skin with soap and water. Wash clothing before reuse.
  • Eye Contact: Immediately flush eyes with water for 15 minutes. Get medical attention.
  • Note to Physician: If patient's immune system is compromised, request antibiotic sensitivity chart from Alken-Murray Corporation by e-mail:


5. Fire Fighting Measures

 Flash Point: NA

Flammability Limits: NA

Autoignition temp: NA

Fire Extinguishing Media: Water spray, carbon dioxide, dry chemical

Special Fire-Fighting Information: None

Unusual Fire and Explosion Hazards: None

6. Accidental Release Measures

For small spills, flush to waste treatment sewer (product is biodegradable). For large spills, contain and collect for reuse.

7. Handling and Storage

 Store in cool dry place. Keep container closed when not in use. Avoid storage of product above 110 degrees F and keep from freezing. Keep out of direct sunlight.

8. Exposure Controls/ Personal Protection

  • Ventilation System: Good general ventilation is advisable
  • Personal Respirators (NIOSH Approved): if needed, use respirator to screen out nuisance dust
  • Skin Protection: Long sleeved clothing
  • Eye Protection: Safety glasses if mist is formed indoors.
  • Other Control Measures: Minimize exposure in accordance with good hygiene practice. Avoid handling which would create large amounts of dust.

9. Physical and Chemical Properties

  • Appearance and Color: Colorless to Tan, slightly turbid liquid.
  • Odor: Faint musty odor
  • Solubility in Water: 99%
  • Specific Gravity: 1.0
  • Bulk density: NDA
  • pH: 7.5 - 8.5
  • % volatile by volume @ 21C (70F): Nil
  • Boiling Point: 212 degrees F, 100 degrees C
  • Melting Point: 32 degrees F
  • Vapor Density (Air=1): =water
  • Vapor Pressure (mm Hg): =water
  • Evaporation Rate (Bu. Acetate=1): =water

10. Stability and Reactivity

  • Stability: Product is stable.
  • Hazardous Decomposition Products: None
  • Hazardous Polymerization: will not occur.
  • Incompatibility: Acids, alkalies, other toxic materials may inactivate biological cultures.
  • Conditions to Avoid: Incompatible substances

11. Toxicological Information

  • Toxicological Data: (CFR Title 16)
RTECS: CAS# 7732-18-5: ZC0110000 / Bacterial cultures: 1003657BA/Bacterial cultures:1004274VB / CAS# 56-81-5: MA8050000 / CAS# 1310-73-2: WB4900000 / CAS# 7783-20-2: BS4500000 / CAS# 7487-88-9:OM4500000 / CAS# 10043-52-4:EV9810000 / CAS# 7558-79-4: WC450000 / CAS# 15708-41-5:AH4900000 / CAS# 7631-95-0: QA5075000 / CAS# 10034-96-5:OP0893500 / CAS# 7791-13-1:GG0200000 / CAS# 7446-20-0: ZH5300000 / CAS# 16052-06-5:TM264913/ Bacterial cultures:1004274VB /CAS# 144-55-8: VZ0950000

LD50/LC50 Individual Ingredients: CAS# 7732-18-5: LD50= >90 mL/Kg / CAS# 56-81-5: oral, mouse > 4090 mg/kg; skin, rabbit 500 mg mild reaction 24H, eye, rabbit, mild reaction at 24 H. / CAS# 1310-73-2: skin, rabbit: 500 mg/24H severe; eye rabbit: 50 ug/24H severe / CAS# 7783-20-2: Oral, rat LD50 = 2840 mg/Kg; Oral, mouse LD50 = 640 mg/Kg / CAS# 7487-88-9: Oral, rat LD50 = 1200 mg/Kg; Oral, mouse LD50 = 645 mg/Kg / CAS# 10043-52-4: Oral, mouse LD50 = 1940 mg/Kg; Oral, rat LD50 = 1 g/Kg / CAS# 7558-79-4: Oral rat LD50: 17 gm/kg. Skin rabbit, standard Draize, 500 mg/24H, mild. Eye rabbit, standard Draize, 500 mg/24H mild / CAS# 15708-41-5: Oral, rat LD50 = 5 g/Kg; Oral,mouse LD50 = 5 g/Kg / CAS# 7631-95-0: Oral, rat LD50 = 4 g/Kg / CAS# 10034-96-5: Oral,rat LD50 = 2150 mg/Kg / CAS# 7791-13-1: Oral, rat LD50 = 766 mg/Kg / CAS# 7446-20-0: Oral, rat LD50 = 2150 mg/Kg; Oral, mouse LD50 = 2200 mg/Kg / CAS# 16052-06-5: Oral, rat LD50 = > 5 g/Kg / CAS# 144-55-8: Oral, mouse LD50 = 3360 mg/Kg; Oral, rat LD50 = 4220 mg/Kg

  • Carcinogenicity:
ACGIH-A3 - Molybdenum soluble compounds (sodium or ammonium) "confirmed animal carcinogen", but is NOT listed by IARC, NIOSH, NTP or OSHA lists.
Other ingredients are NOT found on any lists published by ACGIH, IARC, NIOSH, NTP or OSHA

12. Ecological Information

  • Environmental Fate: This product is not likely to bioconcentrate. If very high concentrations are released into a waterbody, oxygen levels may decrease in the immediate area.
  • Environmental Toxicity: This material has a low potential to affect aquatic organisms, secondary waste treatment microorganisms, and the growth of some plants. Baking soda is approved by the FDA as a food additive under 21CFR172.510 and FEMA #321.

13. Disposal Considerations

RCRA Hazard Class (if discarded): Neat, unused product is not considered a hazardous waste, according to RCRA regulations 40 CFR 261. . For proper disposal of used materials, an assessment must be completed to determine the proper waste management options permissible under applicable rules, regulations and/or laws governing client's location. Use until less than 1 inch remains in the container. Disinfect container with a 1% sodium hypochlorite solution, triple rinse with clean water, and pour rinse water to sewer. Then remove or deface product label to avoid confusion if pail or drum is later refilled. Clean, empty containers should be recycled or disposed of through an approved waste management facility.

14. Transportation Information

  • DOT Proper Shipping Name: Not regulated as hazardous by DOT
  • DOT Hazard Class: None
  • DOT Label: Class 60 (item 20400) "Bacteria, sewage or industrial waste treating, in dry or liquid carrier, with or without enzymes in bulk, bags, barrels or pails" Alken Clear-Flo 1400

15. Regulatory Information

  • TSCA:  (40CFR 710) All chemical ingredients are listed on the TSCA 8(b) inventory. None of the ingredients are on the Health and Safety Reporting List, are under Chemical Test rule, are listed under TSCA Section 12B, or have a SNUR under TSCA
  • CERCLA Reportable Quantity: This product does NOT contain ingredients that have Reportable Quantity (RQ) requirements under US 40 CFR 117.302

    Section 302/304 Extremely Hazardous Substances: Hazardous ingredients are all below de minimus concentrations

    Section 311 Hazard Categorization: Acute, chronic

    Section 313 Toxic Chemicals: This material contains sodium hydroxide (CAS 1310-73-2) which is subject to reporting requirements of Section 313 or Sara Title III and 40 CFR Part 373. 1,000 lb Final RQ (454 Kg). Product has NEUTRAL PH, cancelling reporting requirements.

  • Clean Air Act: This formula does not contain any hazardous air pollutants or ozone depletors.
  • Clean Water Act: This product contains CAS # 1310-73-2, sodium hydroxide, a chemical that CWA lists as a "hazardous substance", but CWA does NOT list ANY product ingredient on their "Priority Pollutant" or "Toxic Pollutant" lists.
  • STATE RIGHT-TO-KNOW LAWS: Glycerine, CAS# 56-81-5, representing < 1% of this formula is listed on the following RTK lists: Massachusetts, Minnesota, Pennsylvania, and Rhode Island. Sodium hydroxide, CAS 1310-73-2, comprising < 1% of this product can be found on the following RTK lists: California, New Jersey, Florida, Pennsylvania, Minnesota & Massachusetts. Other ingredients are not listed on any of the following state right to know lists:California, Connecticut, Florida, Illinois, Louisiana, Maine, Maryland, Massachusetts, Minnesota, New Jersey, New York, Pennsylvania, Rhode Island or Tennessee.
  • Not subject to California Proposition 65 labeling requirements
  • Canadian WHMIS Classification: Class D2B (other toxic effects,applied to ALL bacteria,even BS Class 1, on the DSL list, like the strains in this formula)
  • Canadian DSL: All ingredients comply with Canadian DSL list. Product is authorized for sale in Canada.
  •  Canada's Ingredient Disclosure List: Sodium hydroxide, CAS 1310-73-2, comprising < 1% of this product IS listed on the CIDL, but other ingredients are "NOT CONTROLLED". . This MSDS has been prepared according to the hazard criteria of the Controlled Products Regulations (CPR) and the MSDS contains all of the information required by the CPR. .

16. Other Information

Reason for Revision: Routine review & Revision

Alken-Murray Corporation provides the information contained herein in good faith, in compliance with the Occupational Safety and Health Act of 1970, but makes no representation as to its comprehensiveness or accuracy. This document is intended only as a guide to the appropriate precautionary handling of the material by a properly trained person using this product. Alken-Murray warrants that this product is of merchantable quality. The implied warranty of fitness for a particular purpose is limited to the extent the products are used for the purpose or uses described on the product's label or in any written instructions or materials distributed to the buyer by Alken-Murray and is hereby disclaimed should buyer use the products in a manner inconsistent with this uses or purposes described therein. In no event shall Alken-Murray be liable for any consequential, exemplary, or incidental damages incurred by buyer even if it has been advised of the possibility of such damages.

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