September 17, 1989

Mr. Jon D. Luce, Special Services Manager
Alken-Murray Corporation
(our old address was listed here)

Re: Alken Clear-Flo® 1200, Algal Bloom, Turbidity and Mysidopsis Shrimp Bio-Assay Testing

Dear Mr. Luce:

We have completed testing of Alken Clear-Flo® 1200 with successful results. Three 25,000 gallon tanks containing seawater were treated with 2 oz. each of Alken Clear-Flo® 1200. All three tanks contained excessive algal bloom. This bloom would normally be desirable but it is unpredictable and, overnight, the life-giving green algae can "crash", turning a yellow color and release a number of lethal toxins into the water. Commercially, the presence of algal bloom, especially in shrimp and salmon production, is a serious risk. Tank 1 contained fluke. Tank 2 contained lobsters. Tank 3 contained shrimp.

At the end of the first week, all three tanks exhibited an approximate 25% reduction in the algal bloom, verified by turbidity testing. All three tanks were again treated with a 2 oz. dosage. At the end of the second week, they were again treated with a further reduction of algae noted. At the end of the third week they were again treated with a 2 oz. dosage. At this time the tanks were virtually clear of excess algal bloom. No harmful effects were noted in any of the species and the product is successful in treating for algal bloom.

We are especially excited about the sludge reduction capabilities of this product for use in large scale shrimp, trout, and salmon production. The fecal discharge is tremendous in these species which have been hybridized for fast growth metabolisms. High density stocking and feeding methods employed result in serious organic effluent discharges in these operations. Your product's ability to reduce sludge by 30-60% is valuable to these facilities. We are currently introducing Alken Clear-Flo® to our commercial facilities in Bonaire and Venezuela.

Please note, that when any bacteria is employed, an oxygen demand is created, since bacteria are living organisms and require oxygen in their life cycle. We have conducted Bio-Assay Tests with Mysidopsis Shrimp and have found no harmful effects and consider this product to be quite safe in our aquatic systems. The only precaution I consider necessary is to maintain oxygen levels at the saturation point to ensure adequate oxygen for both the bacteria and the species being produced.

Sincerely yours,

Robert J. Valenti, Ph. D.
Director

RJV/jz