Abstracts of CASE STUDIES

Alken Clear-Flo®, Alken Enz-Odor® & Alken Nu-Bind

This is a sampling of pollution problems we have solved in the past. We are constantly pushing the envelope of technology, creating new formulas and new systems to solve a wide variety of pollution problems. Do not assume that we cannot solve a situation just because it is not on this list, we specialize in custom solutions. When the other companies give up...call on Claudia, Valerie & Ken.


Aquarium & General Aquaculture

1) Clear-Flo 1200 with photos at Marineland, Florida (Marineland1)

  • Letter dated 9/92 discusses success with Clear-Flo 1000 & 1200 in a koi pond and fresh fish tanks.
  • Letter dated 4/93 referencing drain and waste treatment applications

2) Clear-Flo 1200 with photos at Florida Aquaculture Convention hotel in 1992 (Orlando1)


3) Dr. Robert Valenti (Valenti.CXI) results of testing of Clear-Flo 1200 in 1989 in 25,000 gallon seawater fish tanks is demonstrated with before and after photos which show improved water clarity and reduced suspended solids, resulting from microbial removal of excess nutrients in the water, with no harm to flukes, lobsters or shrimp in the tanks.


When Clear-Flo® 1100 was used for transporting Mahi-Mahi eggs from Florida to Monaco, survival rates improved from 25% to 95%.

5) Toxicity testing of Alken Clear-Flo® 1006, revealed no toxicity to Pimephales promelas at 10 to 15 times our shock dosage (5 times normal first dose). Request copy of this report from Valerie.


1) Clear-Flo 1000 & Clear-Flo 1200 reduce suspended organics, algae, and Cyanobacteria in shrimp farm with photos (Shrimpfarm 1)

2) Photos of Latin American growing ponds with Clear-Flo 1200, including dosage rate used for results obtained. (Shrimpfarm 2a)

3) Photos of Latin American reservoir using Clear-Flo 1200, including dosage used by client. (Shrimpfarm 3a)

4) "Use of Beneficial Bacteria in the Larval Rearing of Shrimp Penaeus schmitti", by Elvira Alfonso, Elpidio Beltrame, Edemar R. Andreatta, Alitiene Lemos y Jair Quaresma, sponsored by the Centro de Investigaciones Marinas, Universidad de La Habana, Cuba and Laboratorio de Camaroes Marinhos, Centro de Ciencias Agraras, Universidade Federal de Santa Catarina, Brasil in 1994.

English Abstract: Recently some live organisms have appeared in the market, designed as waste degraders for Aquaculture. The effect of two products (Alken Clear-Flo 1000 and Alken Clear-Flo 1200) on the larval rearing of the white shrimp Pennaeus schmitti was tested. This species is commercially cultured in Brazil and Cuba. Dosages were evaluated, also the oll of the products on the water quality and the possible control of the gut epithelium scaling syndrome of the animals, known as "Bolitas" were studied. In order to analyze the results , the following have been considered: survival, metamorphosis rate, larval quality and size of the postlarvae. It was concluded that the tested products can be used even since Protozoea l. It was possible to reduce the daily water exchange from 100% to 30% in the larval rearing process, besides avoiding the presence of the gut epithelium scaling syndrome from the larvae. The products can optimize the production of P. schmitti postlarvea. Click HERE for the Spanish version of this study.

5) "The Effect of Alken Clear-Flo 1200 used in Grow-Out Ponds of Penaeus japonicus" by Li Hui-Rong, YuYong, Ji Wei-Shang and Xu Huai-Shu, of the College of Marine Life Sciences, Ocean University of Qingdao, China 266003, in 1999.

Synopsis of results: This study demonstrated reduced ammonia levels over controls and increased size, in overall length, width, and weight of the shrimp culture grown when officially expired product was applied. The liquid CF 1200 was NOT supplemented with its usual CF 1001 dry supplement, and did not show a scientifically significant difference in the COD levels versus the control ponds, although both maintained low levels. Click HERE for full length study with diagrams and charts.




Ammonia reduced, sludge degraded by 10 cm/week, and mortality rate reduced by 66% in French trout raceways with Clear-Flo 1200. With photos. (fishfarm1)


1) Chinese eel farm used Clear-Flo 1200 to decreased water changes, off-flavor and hydrogen sulfide formation - with photos (eelfarm1)

2) Photos demonstrate the water quality when Clear-Flo 1200 is used for eel growing (eelfarm4)

3) Since eels absorb 60% of their oxygen through their skins, they are exceptionally sensitive to polluted water. Clear-Flo 1001, 1400 & 1200 decreased the death rate from 50 to 100 eels a day to 5 to 7 eels a day (improved by 93%). Photos illustrate Korean eel farmers at work. (eelfarm5)


1) Prevention of "new tank syndrome" in Korean Koi farms in with the nitrifiers and waste degraders in Clear-Flo 1100, 1200, & 1400 are illustrated with photos from 1993 (Koifarm1)

2) A biofilter, seeded with Clear-Flo 1200 is illustrated in a photo, along with "before" and "after" water samples. (Koifarm2)


Use of Clear-Flo 1200, 1400 & 1100 on an alligator farm is discussed along with photos. (alligator1)


After 22 days of treatment with Clear-Flo 1200 in their alligator and animal ponds and aquariums, aMexican zooexperienced an 84% reduction of BOD and COD, suspended solids decreased by 93%, TKN reduced by over 50%, oxygen levels increased from 0.1 ppm to 2.0 ppm, water clarity was improved by 26%, pH was increased from 6.8 to 7.5, and sediment was decreased from 2.0 to 0!!Click here for Spanish language version of study.


Photos demonstrate the results of frogs grown in Brazil in 1994 with the aid of Clear-Flo 1200 to maintain clean water. Dosages used by the client are listed. (FrogFarm1)

River Remediation & Sludge Removal

1) The scientific report of the 1992-93 remediation of the Moulin Vert Canal using Clear-Flo 1200 for 3 months. (Arnaud.CXI) This report details the application methods, the monitored sludge reduction, and improved water quality. Ammonia was reduced from 0.02 ppm to 00.0 ppm, COD was decreased by 84%, BOD was decreased by 74%. Toxicity tests were performed and proved that there was no detectable toxicity during the treatment. 1500 cubic meters of sludge was degraded during the 3 months. Bioseeding with Clear-Flo 1200 restored the canal's capacity for self-purification as it continues to mineralize sludge, several years after treatment was stopped. Diagrams and photos illustrate this study.

1993 cleanup of a stretch of the Kunming River in China with Clear-Flo 7018, 1200 & 7000 is demonstrated with photos. The anoxic water was so heavily laden with suspended organic waste from fertilizers, animal droppings, fish-farming by-products, septic tank seepage, industrial wastewater and direct disposal of raw garbage that the eutrophic river emitted an unbearable stench. Ammonia and hydrogen sulfide were decreased, sludge was broken up, and as a byproduct of hydrogen sulfide oxidation, free oxygen began to increase. (ChinaRiver1)

The remediation of the River Sorgue in France in 1993 with
Clear-Flo 1200 is illustrated with photos and a newspaper article. BOD and COD were decreased, algal blooms disappeared, and the fish returned within 2 months. A picture of this river is on our HOME page.

4) A Taiwan shrimp farm pond bottom conditioning with Clear-Flo 1200, in 1992, is illustrated with photographs. When the 2 months normally allowed for soil remediation between cycles is decreased, the total annual shrimp production is increased. (Shrimpfarm4)

Before and After photos illustrate how use of Clear-Flo 1000 & 1200 removed the sulphurous bacteria from the sludge in Eelfarm pond bottom in Shinto, China in 1993. (Eelfarm3)

6) "Sludge Removal" uses and photos to illustrate the technique of shrimp pond bottom remediation. (Sludge_Removal.CXI)

General Ponds & Lakes

1) This synopsis of an article written in the Va. Lake Association in 1993, describes the successful treatment of a 7 acre fresh water lake in Virginia Beach, VA with Clear-Flo 1000. By 1998, Sylvan Lake's resident strains of algae had developed an "immunity" to Clear-Flo 1000, finding alternative routes to garner nutrition. A switch to treating the lake with Clear-Flo 1002 solved the problem, restoring clean sparkling water. (Sylvanlk.897)

The remediation of 3 lakes in sequence, at Boxwood Farm, was accomplished with treatments of Clear-Flo 1200, applied to the top lake only, creating visible changes in 2 weeks. Ammonia decreased from 2.5 mg/l to 0.4 mg/l and photos show water so clear it could be mistaken for bottled drinking water! Treatment for this farm is supervised by Mid-County Farm Supply, one of Alken-Murray's earliest distributors for the Clear-Flo® product line. (Boxwood1)

Story of the remediation of Lake Gaskin, surrounded by a commercial development, using Clear-Flo 1000 & 1100 to decreasee ammonia and odors. Photo illustrates the difference in clarity between water samples taken before and after treatment (illustrates how well newsprint is read through the treated water) and shows the difference in water clarity in the actual lake. (LakeGaskin1)

The story in the "Clean Water/Lake Management" Alken-Murray three-fold brochure gives a synopsis of the treatment of a large lake, a large pond and "turtle pond" in Central Park, New York in the summer of 1992. A longer version of the study gives full details of treatment applications (lower doses now because of increased product concentration and improved balance) with Clear-Flo 1200, 1000, and 1001. The "Lake" showed Secchi disk reading improved by 78%, suspended solids decreased by 77%, and BOD decreased by 60%. "Turtle Pond" Secchi disk readings were improved by 78%, suspended solids decreased by 70%, and BOD decreased by 72%. The "Pond" Secchi disk readings improved by 37%, suspended solids decreased by 62%, BOD decreased by 52%.

5) Filamentous "blue-green algae" aka Cyanobacteria discouraged by Clear-Flo 1200 with Supplement C in 1997 case study from lakes belonging to the City of Gaithersburg, Maryland. Account handled by Alken-Murray Distributor, Wildlife Habitats.

6) In 1991, CF 1000 and 1200 were used to restore clean water to the 12 acre lake at Merry Oak Farm in Warrenton, VA, home to crappy, bass, swans, Canadian geese and other migratory fowl. Farm manager, Richard Mayberry, said "...in six weeks, we cleaned up our lake completely with Alken Clear-Flo. We've got absolutely no algae! Riders can water their horses here in safety because we don't use dangerous chemicals in the water." Below is a picture of the appearance of the water, before and after.

picture illustrates the change from untreated water to treated water in laboratory flasks


Country Club Ponds

1) The story of an American golf club's experience with Clear-Flo 7000 and 7110 in 1991. Odors were reduced in 10 days, turbidity decreased by 65% within a month. After 2 months of treatment, turbidity was reduced by 95%, algae blooms had been avoided, and there were no unpleasant odors. (Golfclub)

2) An Oklahoma country club used Clear-Flo 1200 and 7118 (OklahomaCC.CXI) to clear up murky water in 1994, illustrated with photos.

A Midwestern golf club used Clear-Flo 1200 & 1001 to eliminate a mat of algae so thick it looked like you could walk on it.

In 1998, Guadalajara Country Club used Clear-Flo 7000 to discourage most surface algae, but were unhappy with the green color that persisted in the water. After consulting with Claudia Velasco, VP Management of Alken-Murray, the treatment was altered. One small dose of polymer was added to a small dose of Clear-Flo 1001 to instantly clear the water. Follow up treatment with Clear-Flo 1200 with its Clear-Flo 1001 supplement has maintained clean water for this pond. BOD decreased by 97%. COD decreased by 85%. SST decreased by 98%, and phosphates decreased by 69%.Click here for Spanish version of study.


Manure Management

1) Application methods and dosage for Clear-Flo 7000 is described for a chicken egg laying factory to reduce odors and improve the quality of the digested waste. (Chickenfarm1.cxi)

A solution for odors in chicken manure prior to processing into fertilizer using Clear-Flo 1100 was outlined in 1992. (Chicken.02)

In 1997, Alken Clear-Flo® 7000 was used in four in-line chicken farm lagoons in Brazil. Tests by the regulatory agency certified laboratory demonstrated over 87% efficiency reducing BOD from an average of 1,166 ppm to 150 ppm. Excellent "before" and "after" photos.(Chicken3.CXI)

Information and illustrations of Clear-Flo 7000 in use in animal producing farms (PigFarm1.DJC)

A case study of Clear-Flo 7000 used on a brand-new Oklahoma pig farm in 1993, with application illustrations and photos. Even with the ventilators closed, the only odor evident was straw and animal feed. (PigFarm2.DJC) .

6) Dosage rates for use of Clear-Flo 7000 and Clear-Flo 4003 in a rabbit factory is described. (Rabbits.196)

7) Independent testing of Alken Enz-Odor® 5 compared with 35 competing manure pit additives. Test conducted by Purdue, sponsored by NPPC.

Clear-Flo Helps Sausage Manufacturer Meet Discharge Permit in Two Weeks

One of Europe's largest sausage manufacturers, faced fines in 2002, for discharging up to double their permitted level of BOD and up to 26% above permitted COD levels. AM Greek distributor, BioBest used a blend of Alken Clear-Flo products (CF 7000, 7004, 7008 and 4003) by three weeks and continuing good results with lower levels of treatment for three months.

Ethylene Glycol Treatability Study

In 1993, a laboratory treatability study was performed to demonstrate that ethylene glycol could be degraded at 50F, by Clear-Flo® 7030, at an average rate of 270 mg/L/day (up to 350 mg/l/day) after a 5 day lag period. Concentrations up to 15% can be handled at higher temperatures. Illustrated with color graphs. (EthyleneGlycol.DJC)

Grease in Septic Systems

In 1994, Alken Clear-Flo 4000 was proven effective in a 1 year septic system study, testing results in 7 residences, cuncurrent with laboratory batch reactor tests which simulated shock loading with detergents and BOD. (Septic.wpd)

Hydrogen Sulfide Cleanup

1) In August of 1991, a Latin American resort used Clear-Flo 7100 and 7108 in the gravity mains of their collection system to reduce hydrogen sulfide concentrations below the odor threshold most of the time. A color graph illustrates the results before and after treatment. (Resort.CXI)

2) Domestic RBC plant and collection system with additional grease and oil buildup problem, treated with Clear-Flo 7108/4103 in the collection system. Plant problems treated with Clear-Flo 7008/4003 in summer and Clear-Flo 7018/4003 in winter with a small amount of polymer added to the gravity settler. (Collect.697)

3) Clear-Flo 7008 used to eliminate hydrogen sulfide while Clear-Flo 4003 used to degrade oils and improve mixed liquor solids control and COD reduction.

(also applicable to other edible plant oils - original case study LOST - no additional info.)


In 1991, a plant processing over 200,000 barrels of beer a month, nearly 0.5 MGD wastewater with low nutrients and high phenolic sanitizers used Clear-Flo 7002. Effluent TSS decreased from nearly 400 mg/l to around 25 mg/l. Effluent BOD decreased from 60 mg/l to 15 mg/l. Turbidity reduced from 200 ntu to around 10 ntu. and polymer dosage reduced from 800 mg/l to less than 100 mg/l. With graphs and plant diagram. (Brewery.CXI)

Powdered Milk Production

Liconsa in Mexico - Application of Alken Clear-Flo® 4000 almost doubled TSS and VSS reactor removal rates, 27% improved clarity of effluent, COD reduced by 52%, and solids settling reactor rate improved by nearly 76%. Click HERE for Spanish language version.

Drain lines

1) Psychiatric institute in Virginia used Clear-Flo 4100 to keep drain lines clean. - 1992

2) Midwestern Resort used Clear-Flo 4100 to aid grease trap, drain lines - 1991

3) Laboratory tests show waste degrading performance of Clear-Flo 4000, even in the presence of high concentrations of a popular laundry detergent.


For more grease-degrading case studies, see the following:

  • Rabbit factory used CF 4003 ( study above)
  • Domestic RBC used CF 4003 (see Hydrogen Sulfide studies, above)
  • Eel and shrimp processing plants used CF 4003 (see Animal Processing Plants, above)
  • Powdered Milk production used CF 4000 (see above)
  • Haicare products mfg. used CF 4000 (see below)
  • Dairy used CF 4003 (see below)

Animal Processing Plant Waste

1) Clear-Flo 4000 and 7000 as used in 1993 by a Shinto, China Eel farm processing plant is discussed - photos ( EelFarm2)

2) Thailand shrimp processing plant improved effluent with Clear-Flo 4003 in the anaerobic lagoon & Clear-Flo 7000 in the aerobic lagoon. BOD effluent decreased from 100 to 30 mg/l while SS decreased from 120 mg/l to 50 mg/l (Shrimpfarm5.)

Ammonia Reduction in a Waste Treatment Facility

A Virginia public treatment plant used Clear-Flo 7110 in 1991 to decrease effluent ammonia concentrations by 75%. Illustrated with color graphs. (7110Rem)

Factory Discharge to Municipal Waste Treatment Managed

1) A Southwestern US hair care products manufacturer had significant problems with dyes and grease overloads on an under-designed system. They were heavily fined for discharges up to 40 times the permitted levels. TPH was decreased from an average 500 ppm to an average 12.5 ppm (a decrease of over 97%) with Clear-Flo 4000 and 7100 treatment, despite a too-short retention time. (Haircare.697) .

2) In 1992, a dairy's activated sludge system treated with Clear-Flo 4003 to degrade dairy byproducts and Clear-Flo 7002 to degrade the CIP sanitizers. Settling improved from zero to 100-300 ml. (Dairy)

3) A refinery decreased BOD by 71%, with concurrent decreased phenol and sulfide levels while treating with Clear-Flo 7002/7008. (Refiner1.wpd)

4) A metal plating operation's discharge caused severe operations problems for a plant in 1992 until they began using Clear-Flo 7000 and Clear-Flo 7018 (today this blend is available as CF 7001). Effluent BOD reduced from 100 mg/l to below 15 mg/l. Effluent TSS reduced from 98 mg/l to 10 mg/l. With graph.(MWT7018)

5) Complete operations of a paper & pulp mill improved in 1989-90 with Clear-Flo 7008 & 7018. (Today the replacemtent formula is the improved CF 7008.) TSS decreased by and BOD violations eliminated. Illustrated with color graphs and diagrams. The average suspended solids was decreased by 50%. Recovery after upset caused by aeration equipment failure is also illustrated. (PaperMill.CXI)

6) Clear-Flo 7015 handles Nocardia and foaming problems for textile chemical manufacturer. Other foaming problems can be handled by Clear-Flo 7004. Filamentous algae and foaming caused by Nocardia is illustrated with a photo. (NocardiaFoam1.CXI)

Phenolic compounds in effluent from a tar processing plant reduced from 1500 mg/l to 0.1 mg/l with Clear-Flo 7002, augmented with Clear-Flo 7018 during cold weather. (tar.697)

In 1984, Clear-Flo 7002 and 7004 were used to assist start-up in a textile chemical manufacturing plant.

A coke reduction plant used Clear-Flo 7002 and 7008 to reduce cyanide levels from 2000-4000 ppm to 400 ppm and sulfide levels from 3000-11000 to less than 400 ppm. (cokeplnt.697)

Clear-Flo 7008 & 7018 cleanup ortho-dichlorobenzene spill at organic chemicals manufacturing plant .

Citrus Process Wastewater & Citrus-Extract Chemical

1) Custom Micro-Consortium Degrades Citrus Extract Chemical Cleaner & Its Extracted Pollution.. The story of Solutions Special X3™.

2) Citrus process wastewater BOD improved with Clear-Flo 7008.

Petrochemical Company

Clear-Flo 7001/7002 used to reduce effluent of toluene and phenols discharged to local river (phenol.697)Although this study was performed with CF 7001 and CF 7002, today we would prescribe our improved new product CF 7008

Paper Mill Discharging to a River

Mexican pulp and paper mill, Industrias Centauro, tried 5 competing products with no success prior to working with Alken-Murray distributor DEPQUI. Once their faulty aerators were fixed and/or replaced, our Clear-Flo 7005 and Clear-Flo 7008 (warm weather) and Clear-Flo 7026 (cold weather), assisted by a small application of polymer, brought this plant into compliance with their discharge permit, reducing BOD, COD, TSS and dissolved solids to meet the discharge limit for river discharge.

Wood Preserving Waste

A Michigan wood preserving company achieved successful treatment results for their wastewater, using a bioremediation custom blend, combining Alken Clear-Flo 7001 & 7002 with a pair of Pseudomonas aeruginosa strains, selected to target naphthalene and anthracene and one strain of Pseudomonas fluorescens specializing in biodegradation of PAH's, especially phenanthrene, and fluoranthene. Chart illustrates reduction of phenol, pentachlorophenol, napthalene, anthracene, fluorane, and carbazole. (WoodWasteReduction.CXI)


1) Polypropylene manufacturer's facultative lagoons treated with Clear-Flo 7008 obtained a 46% decrease is BOD/COD levels, keeping the company discharge within permitted levels. (lagoon2.697)

2) Virginia municipal waste treatment plant's polishing lagoons were treated in 1991 with Clear-Flo 1000, reducing odors and algae within one week. Photos illustrate before and after treatment. (GooseCreek.CXI)

Alken Enz-Odor®

1) The October-November 1991 issue of The Florida PET Review contains a favorable review of the performance of Alken Enz-Odor® (original blend) tells how the product performed for the reviewers.

I Love Cats Magazine carried a favorable review of the original formula of Alken Enz-Odor® in July 1991.

Letter from Wyld-Kats Cattery recommending Alken Enz-Odor® 4 for cat litter pans.

4) Letter from T. Grace Atkinson recommending Alken Enz-Odor® 3 for female cat odor.

5) Alken Enz-Odor® vs. The Herd of Skunks. Our newest testimonial, case study for this product is a zinger! 6 skunks invaded the basement and under-deck area of a house FOR SALE!! Humane trapper, Jim Reavis, removed the skunks and used Alken Enz-Odor® to remove the odor!! Read this new story.

6) Independent testing of Alken Enz-Odor® 5 compared with 35 competing manure pit additives. Test conducted by Purdue, sponsored by NPPC.

 7) Letter from Sheila Ezratty 05/23/01 recommendingthe new improved formula Alken Enz-Odor® 4 for cat litter pans. Ms. Ezratty has been a long-time user of Alken Enz-Odor® (original blend).

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